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Ninhydrin and ionic liquid reagent mixture and method for the visualization of amino acids and peptides

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Класс G01N33/00 Исследование или анализ материалов особыми способами, не отнесенными к группам  1/00

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Класс C07C49/00 Кетоны; кетены; димеры кетенов; хелатные соединения кетонов


Классы МПК:G01N33/00 Исследование или анализ материалов особыми способами, не отнесенными к группам  1/00
C07C49/00 Кетоны; кетены; димеры кетенов; хелатные соединения кетонов
Автор(ы): Schulz, Michael (Darmstadt, DE)
Hauck, Heinz-emil (Gross-Umstadt, DE)
Патентообладатель(и): Merck Patent Gesellschaft (Darmstadt, DE)
Приоритеты:
подача заявки
03.07.2007
публикация патента
28.12.2010

РЕФЕРАТ (Abstract)

The invention relates to a method and reagent mixture for the staining and thus visualization of amino acids, peptides and similar compounds, in particular after separation by means of thin-layer chromatography. The staining is carried out using ninhydrin for the detection of amino acids, peptides or proteins in combination with at least one ionic liquid.
Полный текст Патента US 7858376 + PDF


ФОРМУЛА ИЗОБРЕТЕНИЯ (CLAIMS)

The invention claimed is:

1. A reagent mixture for the detection of amino acids, peptides or proteins comprising: a first reagent comprising ninhydrin; and at least one other reagent comprising at least one ionic liquid selected from 1-pentyl-3-methylimidazolium chloride, 1-hexyl-3-methylimidazolium chloride, 1-heptyl-3-methylimidazolium chloride, 1-octyl-3-methylimidazolium chloride, 1-nonyl-3-methylimidazolium chloride, 1-decyl-3-methylimidazolium chloride, trihexyltetradecylphosphonium chloride, ethyldimethylpentylammonium bromide, ethyldimethylpropylammonium chloride, 1-methyl-1-octylpyrrolidinium chloride, n-butylpyridinium chloride, n-octylpyridinium chloride, 4-methyl-4-pentylmorpholinium bromide, and 1-methyl-1-pentylpiperidinium bromide.

2. The reagent mixture according to claim 1, wherein said reagent mixture comprises 0.01-10% by weight of ninhydrin and 0.1-10% by weight said at least one ionic liquid.

3. The reagent mixture according to claim 1, wherein a) said first reagent comprises 0.01-10% by weight of ninhydrin in at least one solvent, and b) said at least one other reagent comprises 0.1 to 10% by weight of said at least one ionic liquid in at least one solvent.

4. The reagent mixture according to claim 3, wherein said first reagent contains 0.1-1% by weight of ninhydrin.

5. An article comprising said reagent mixture according to claim 3, and at least one TLC plate.

6. An article comprising said reagent mixture according to claim 1, and at least one TLC plate.

7. A method for the visualization of amino acids, peptides and/or proteins, comprising: treating amino acids, peptides and/or proteins with a reagent mixture according to claim 1.

8. A method for the analysis of a sample comprising amino acids, peptides and/or proteins, said method comprising: a) chromatographic separation of said sample on a thin-layer chromatography plate (TLC plate); b) staining of the resultant separated sample on said TLC plate with said reagent mixture according to claim 1.

9. The method according to claim 8, wherein said TLC plate is a plate having a nonpolar phase or a cellulose phase.

10. The method according to claim 8, wherein said TLC plate is heated in step b) to a temperature between 50 and 200° C. for 0.5 to 10 minutes after staining with said reagent mixture according to claim 1.

11. The method according to claim 8, wherein said first reagent comprises 0.01-10% by weight of ninhydrin in at least one solvent, and said at least one other reagent comprises 0.1 to 10% by weight of said at least one ionic liquid in at least one solvent.

12. The method according to claim 11, wherein said first reagent comprises 0.1-1% by weight of ninhydrin.

13. The method according to claim 8, wherein said first reagent and said at least one ionic liquid are together in a liquid reagent.

14. The method according to claim 13, wherein said liquid reagent comprises 0.01-10% by weight of ninhydrin and 0.1-10% by weight of said at least one ionic liquid.

15. A method for the analysis of a sample comprising amino acids, peptides and/or proteins by chromatographic separation, said method comprising subjecting said sample to chromatographic separation on a thin-layer chromatography plate (TLC plate), where the mobile phase used during said chromatographic separation comprises said reagent mixture according to claim 1.

16. The method according to claim 15, wherein said TLC plate is a plate having a nonpolar phase or a cellulose phase.

17. The method according to claim 15, wherein after chromatographic separation said TLC plate is heated to a temperature between 50 and 200° C. for 0.5 to 10 minutes.


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